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KMID : 0364320150400030035
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Journal of East-West Medicine
2015 Volume.40 No. 3 p.35 ~ p.45
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Inhibitory effect of Bambusae Caulis In Liquamen on pro-inflammatory mediators in vitro
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Kim Seon-Young
Jung Dae-Hwa
Back Young-Doo
Park Sang-Mi
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Abstract
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In the present study, we have demonstrated the anti-inflammatory effects of Bambusae Caulis In Liquamen (BCIL) in macrophage cell line. To investigate the anti-inflammatory activity, we examined the effects of the lipopolysaccaride (LPS)-induced production of nitric oxide (NO), pro-inflammatory cytokines and prostaglandin E2 (PGE2) in a murine macrophage cell line Raw 264.7. The Raw 264.7 cells were cultured in DMEM + serum medium for 24 hr. After serum starvation for 24 hr, the cells were treated with BCIL 1, 3, 9 (‰) for 1 h, followed by stimulation with LPS (1 ㎍/㎖) for activation of immune response. After treatment, cell viability was measured by MTT assay, and NO production was monitored by measuring the nitrite content in culture medium. The levels of cytokine were analyzed by sandwich immunoassays. As results, BCIL has an inhibitory effect on the production of NO, PGE2, Tumor necrosis factor-α and Interleukin-6. These results suggest that BCIL can inhibit production of pro-inflammatory mediators and might be a useful source for treatment of acute inflammatory disease.
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KEYWORD
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Bambusae Caulis In Liquamen (BCIL), Inflammatory mediators
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